Supercritical fluid chromatography - Application to trace analysis
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چکیده
Figure 3. High performance capillary SDS-PAGE separation of myoglobin and several of its fragments. Conditions: 400 V/cm, 34 AA, 25 'C, migration distance 20 cm; fused-silica capillaries; 75 Am ID, T-12.5%, c=3.3%. Buffer: 0.1 M Tris-HPO0 (pH=6.9), 0.1% SDS, 8 M urea. Supercritical fluid chromatography (SEC) is fast becoming the method of choice for many separations involving nonvolatile, reactive, and thermally labile compounds. In particular, supercritical car-
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